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Image Search Results
Journal: Frontiers in Cellular Neuroscience
Article Title: Supraphysiological doses of performance enhancing anabolic-androgenic steroids exert direct toxic effects on neuron-like cells
doi: 10.3389/fncel.2013.00069
Figure Lengend Snippet: Immunofluorescence for neurofilament to demonstrate neurite outgrowth in PC12 cells differentiated in NGF in tissue culture, either control treated ( left panel ), treated with 75 μM methandienone ( center panel ) or treated with 75 μM of 17-α-methyltestosterone ( right panel ) (A). Quantification of neurite outgrowth in the three conditions shown in (A) , as determined by cells exhibiting neurites equal to or greater than the length of one cell body, as a percentage of the total number of cells counted ( Y-axis ). Experiments were repeated at least three times and data expressed as mean ± SD ( ** P ≤ 0.01). These AAS demonstrate toxic effects on formed neurite networks in differentiated PC12 (B). Average of the sums of neurite length for identified neurite bearing cells in 10 high-power fields ( Y-axis ). Experiments were repeated at least three times and data expressed as mean ± SD ( ** P ≤ 0.01) (C) .
Article Snippet: 1 × 10 5 PC12 cells per square centimeter growing on poly-D-lysine coated cover slips were differentiated for 5 days in 200 ng/ml of NGF (Promega), followed by treatment with 75 μM of
Techniques: Immunofluorescence
Journal: Frontiers in Cellular Neuroscience
Article Title: Supraphysiological doses of performance enhancing anabolic-androgenic steroids exert direct toxic effects on neuron-like cells
doi: 10.3389/fncel.2013.00069
Figure Lengend Snippet: PC12 cells were control treated ( left panel ) or treated with 75 μM methandienone ( center panel ) or 75 μM 17-α-methyltestosterone ( right panel ) for 48 h and a vitality assay was performed. Green cells are vital; cells in early cell death are yellow; orange indicates late cell death (A). Vitality status expressed as percentage of total live cells counted in 10 high power fields ( Y-axis ) reveals that methandienone and 17-α-methyltestosterone induce cell death in PC12. Experiments were repeated at least three times and data expressed as mean ± SD ( * P ≤ 0.05) (B) .
Article Snippet: 1 × 10 5 PC12 cells per square centimeter growing on poly-D-lysine coated cover slips were differentiated for 5 days in 200 ng/ml of NGF (Promega), followed by treatment with 75 μM of
Techniques:
Journal: Frontiers in Cellular Neuroscience
Article Title: Supraphysiological doses of performance enhancing anabolic-androgenic steroids exert direct toxic effects on neuron-like cells
doi: 10.3389/fncel.2013.00069
Figure Lengend Snippet: Immunoblots demonstrate decreasing levels of phospho-ERK ( top panel ) in PC12 treated for 48 h with 75 μM androsterone, nandrolone, methandienone, and 17-α-methyltestosterone, compared to control treated cells (A) . Immunoblots for caspase 3 ( top panel ), PARP ( second panel ), and Hsp90 ( third panel ) in PC12, control treated or treated 48 h with 75 μM androsterone, nandrolone, methandienone, and 17-α-methyltestosterone demonstrate increasing levels of the activated fragment of caspase 3 and cleavage of PARP and Hsp90, indications of apoptosis and cell death (B) . PC12 were control treated or treated for 48 h with 50 or 75 μM methandienone ( left column ) or 17-α-methyltestosterone ( right column ). Increasing concentrations of these AAS resulted in appearance of the activated fragment of caspase 3 and cleavage of PARP and Hsp90 in a dose-dependent manner (C) . GAPDH was used as the loading control for all blots ( bottom panels ).
Article Snippet: 1 × 10 5 PC12 cells per square centimeter growing on poly-D-lysine coated cover slips were differentiated for 5 days in 200 ng/ml of NGF (Promega), followed by treatment with 75 μM of
Techniques: Western Blot
Journal: Frontiers in Cellular Neuroscience
Article Title: Supraphysiological doses of performance enhancing anabolic-androgenic steroids exert direct toxic effects on neuron-like cells
doi: 10.3389/fncel.2013.00069
Figure Lengend Snippet: Immunoblots for caspase 3 ( top panel ), PARP ( second panel ) (A) and Hsp90 (B) in PC12, control treated or treated for 48 h with 75 μ M methandienone and 17-α-methyltestosterone alone or with 10 μM hydroxyflutamide. Indicators of apoptosis are decreased in the presence of hydroxyflutamide. GAPDH was used as the loading control for all blots ( bottom panels ).
Article Snippet: 1 × 10 5 PC12 cells per square centimeter growing on poly-D-lysine coated cover slips were differentiated for 5 days in 200 ng/ml of NGF (Promega), followed by treatment with 75 μM of
Techniques: Western Blot